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assumed that the residence time in the impeller region is relatively small and that the remainder of the vessel behaves like a well-mixed tank with a much longer residence time, eq (18-55) can be used to describe the CTD Figure 18-7 also shows the cumulative CTD calculated using eq (18-55), with a mean circulation time of 12 s, for a single circulation in the STR + STR model Comparison of the experimental and calculated cumulative CTDs shows that eq (18-55) is fairly accurate in predicting the experimental CTD, and this is especially the case for the tail of the distributions Therefore, the STR + STR model is well suited to study the effects of cell residence in the poorly oxygenated bulk regions, alternated by residence in the well-aerated impeller region Detailed descriptions of the scale-down models and their operation are provided in Amanullah et al (1993a) and Amanullah (1994).

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fallen to a value of -kTe 12e relative to the plasma potential where the density is no (far from the edge of the plasma). The electron density at the sheath edge is then

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Figure 18-8a depicts the scale-down STR + STR con guration in the laboratory Two interconnected stirred vessels 6 L (28 or 48 L working volumes) and 2 L (12 L working volume) in capacity were used Two variable speed pumps with a maximum estimated pumping capacity of 16 L/min, enabling a minimum mean circulation time of 15 s, were employed to provide the ow between the vessels Dissolved oxygen tension (DOT) was measured and controlled at 5% 05% of air saturation in the smaller vessel by means of gas blending, keeping the total ow of gas constant at 1 vvm Nitrogen was sparged at a rate of 05 vvm into the larger vessel to maintain a DOT level close to zero The mean circulation time (tc ) was varied in the range 15 to 300 s at Va /Vq of 025 and 0.

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43, where Va and Vq , are volumes of the active (small bioreactor with DOT at 5%) and quiescent (large bioreactor with DOT close to zero) zones, respectively In the STR + PFR model (Figure 18-8b), the dissolved oxygen was maintained at 10% by gas blending in the STR The plug ow volume was either 2 or 4 L, resulting in Va /Vq = 05 and 025, respectively The results from both the STR + STR and STR + PFR models were compared against a control batch fermentation with DOT = 10% This control represented the ideal mixing situation in terms of oxygen supply and corresponded in effect to a zeromean circulation time The control experiment yielded maximum values of both nal biomass concentration (644 g/L) and speci c growth, rate ( ) at 031 h 1 .

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birt ean 128

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